Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris . by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides , a recombinant expression plasmid ppic9k - fl was constructed , and integrated into the alcohol oxidase region of the host genome 为了提高外源基因的表达量，我们根据毕赤氏酵母偏爱密码子人工合成了编码fl胞外区156个氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表达载体ppic9k质粒上，构建ppic9k - fl表达质粒。
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